[Frontiers in Bioscience 13, 1050-1061, January 1, 2008] 1050 Clinical significance of a novel single nucleotide polymorphism in the 5’ untranslated region of the Rabphillin-3A-Like gene in colorectal adenocarcinoma

1. Abstract 2. Introduction 3. Materials and methods 3.1. Patients 3.1.1. Prospective CRC samples 3.1.2. Retrospective CRC samples 3.1.3. Patient demographics, clinical and follow-up information 3.1.4. Pathologic features 3.2. Mutational analysis of the RPH3AL gene 3.2.1. RNA extraction, PCR analysis, and DNA sequencing of RPH3AL gene 3.3. Expression of mRNA of RPH3AL 3.3.1. Quantitative real-time PCR analysis 3.4. Genotyping of the SNP at 5’UTR-25 of RPH3AL 3.4.1. DNA extraction from paraffin blocks and frozen tissues 3.4.2. PCR-confronting two pair primer method and sequence analysis 3.5. Analysis of other SNPs in the genomic region of RPH3AL 3.6. Statistical analyses 4. Results 4.1. Mutational status of the RPH3AL gene in prospective specimens 4.2. Genotyping of the SNP at 5’UTR-25 in retrospective specimens 4.3. Correlation between 5’UTR-25 alterations and clinicopathological features 4.4. mRNA expression of the RPH3AL gene based on the SNP at 5’UTR-25 4.5. Linkage disequilibrium of the SNP at 5’UTR-25 of RPH3AL 4.6. Risk of recurrence 4.7. Survival analyses 5. Discussion 6. Acknowledgements 7. References